Reference number
ISO/TS 20131-1:2018
Technical Specification
ISO/TS 20131-1:2018
Soil quality — Easy laboratory assessments of soil denitrification, a process source of N2O emissions — Part 1: Soil denitrifying enzymes activities
Edition 1
2018-07
Technical Specification
Preview
ISO/TS 20131-1:2018
67075
No disponible en español
Publicado (Edición 1, 2018)
Esta publicación se revisó y confirmó por última vez en 2021. Por lo tanto, esta versión es la actual.

ISO/TS 20131-1:2018

ISO/TS 20131-1:2018
67075
Idioma
Formato
CHF 63
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Resumen

This document specifies a laboratory test for characterizing the denitrifying enzyme activities in soils[5]. It globally characterizes the transformation of the nitrate form to the nitrous oxide and dinitrogen forms. This method was first proposed by Reference [5] with the acronym DEA for Denitrifying Enzyme Activities. It is a standardized technique used in numerous scientific studies.

DEA estimates the process of denitrification of fresh soil samples incubated under optimal conditions of substrates (nitrate and carbon sources) and environment (anaerobiosis, controlled temperature) for the denitrification process. The de novo enzyme synthesis is blocked by the use of chloramphenicol. DEA is believed to represent the size of the denitrifying enzyme pool present in the soil sample at the time of sample collection.

This test is performed in laboratory on a composite of sieved samples collected at the plot scale. It can be performed on all types of soils sampled all over the year.

Informaciones generales

  •  : Publicado
     : 2018-07
    : Norma Internacional en proceso de revisión sistemática [90.20]
  •  : 1
     : 12
  • ISO/TC 190/SC 4
    13.080.30 
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