Reference number
ISO 9308-1:2014
International Standard
ISO 9308-1:2014
Water quality — Enumeration of Escherichia coli and coliform bacteria — Part 1: Membrane filtration method for waters with low bacterial background flora
Edition 3
2014-09
Read sample
ISO 9308-1:2014
55832
Published (Edition 3, 2014)
This publication was last reviewed and confirmed in 2019. Therefore this version remains current.
This standard has 1 amendment.

ISO 9308-1:2014

ISO 9308-1:2014
55832
Language
Format
CHF 63
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Abstract

ISO 9308-1:2014 specifies a method for the enumeration of Escherichia coli (E. coli) and coliform bacteria. The method is based on membrane filtration, subsequent culture on a chromogenic coliform agar medium, and calculation of the number of target organisms in the sample. Due to the low selectivity of the differential agar medium, background growth can interfere with the reliable enumeration of E. coli and coliform bacteria, for example, in surface waters or shallow well waters. This method is not suitable for these types of water.

ISO 9308-1:2014 is especially suitable for waters with low bacterial numbers that will cause less than 100 total colonies on chromogenic coliform agar (CCA). These may be drinking water, disinfected pool water, or finished water from drinking water treatment plants.

Some strains of E. coli which are β-D-glucuronidase negative, such as Escherichia coli O157, will not be detected as E. coli. As they are β-D-galactosidase positive, they will appear as coliform bacteria on this chromogenic agar.

General information

  •  : Published
     : 2014-09
    : International Standard under systematic review [90.20]
  •  : 3
     : 10
  • ISO/TC 147/SC 4
    07.100.20 
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 Amendments

Amendments are issued when it is found that new material may need to be added to an existing standardization document. They may also include editorial or technical corrections to be applied to the existing document.

Amendment 1

Edition 2016

ISO 9308-1:2014/Amd 1:2016
70094
Language
Format
CHF 18
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Life cycle

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