International Standard
ISO/TS 19006:2016
Nanotechnologies — 5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line
Reference number
ISO/TS 19006:2016
Edition 1
2016-08
International Standard
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p
ISO/TS 19006:2016
63697
Published (Edition 1, 2016)
This standard was last reviewed and confirmed in 2023. Therefore this version remains current.

ISO/TS 19006:2016

ISO/TS 19006:2016
63697
Format
Language
CHF 129
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Abstract

ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.

The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.

General information

  •  : Published
     : 2016-08
    : International Standard confirmed [90.93]
  •  : 1
     : 21
  • ISO/TC 229
    07.120 
  • RSS updates

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